tau Protein (AA 249-381) (His tag)

Catalog No. ABIN1097616

Product Details

Target: tau (tau Protein)

Protein Type: Recombinant

Protein Characteristics: AA 249-381

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This tau protein is labelled with His tag.

Purpose: Recombinant Human Microtubule-Associated Protein Tau/MAPT-D/Tau-D (C-6His)

Sequence: MQIVYKPVDL SKVTSKCGSL GNIHHKPGGG QVEVKSEKLD FKDRVQSKIG SLDNITHVPG GGNKKIETHK LTFRENAKAK TDHGAEIVYK SPVVSGDTSP RHLSNVSSTG SIDMVDSPQL ATLADEVSAS LAKQLEHHHH HH

Characteristics: Recombinant Human Microtubule-Associated Protein Tau/MAPT is produced with our E. coli expression system. The target protein is expressed with sequence (Gln249-Gln381) of Human MAPT.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: tau (tau Protein)

Alternative Name: tau (tau Products)

Synonyms: DDPAC Protein, FTDP-17 Protein, MAPTL Protein, MSTD Protein, MTBT1 Protein, MTBT2 Protein, PPND Protein, TAU Protein, tau Protein, PHF-tau Protein, microtubule associated protein tau Protein, MAPT Protein

Background: Microtubule-Associated Protein TAU is abundantly expressed in neurons of the central nervous system and less commonly expressed elsewhere, but is also expressed at very low levels in CNS astrocytes and oligodendrocytes. Tau interacts with tubulin to stabilize microtubules and promotes tubulin assembly into microtubules. The C-terminus of TAU binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau acts as a linker protein. When tau is defective, and no longer stabilize microtubules properly, it can result in dementias such as Alzheimer's disease and other tauopathies.
Alternative Names: Microtubule-Associated Protein Tau, Neurofibrillary Tangle Protein, Paired Helical Filament-Tau, PHF-Tau, MAPT, MAPTL, MTBT1, TAU

Molecular Weight: 15.37 kDa

UniProt: P10636