TIM Protein (AA 1-249) (His tag)
TIM
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target See all TIM products
- TIM (Triosephosphate Isomerase (TIM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-249
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TIM protein is labelled with His tag.
- Purpose
- Recombinant Human Triosephosphate Isomerase/TIM (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
- Characteristics
- Recombinant Human Triosephosphate Isomerase/TIM is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Gln249) of Human TPI1 fused with a His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 10 % Glycerol, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- TIM (Triosephosphate Isomerase (TIM))
- Alternative Name
- Triosephosphate Isomerase/TIM (TIM Products)
- Synonyms
- F26H11.7 Protein, F26H11_7 Protein, PDTPI Protein, PLASTID ISOFORM TRIOSE PHOSPHATE ISOMERASE Protein, TRIOSEPHOSPHATE ISOMERASE Protein, triosephosphate isomerase Protein, triosephosphate isomerase Protein, TIM Protein
- Sub Type
- Fusionprotein
- Background
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Triose-phosphate isomerase, also named Triose-phosphate isomerase, TPI and TIM, is an enzyme that catalyzes the reversible interconversion of the triose phosphate isomers dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. TPI has been found in nearly every organism searched for the enzyme, including animals such as mammals and insects as well as in fungi, plants, and bacteria. However, some bacteria that do not perform glycolysis, like ureaplasmas, lack TPI. TPI plays an important role in glycolysis and is essential for efficient energy production. TPI deficiency is an autosomal recessive disorder and the most severe clinical disorder of glycolysis. Triose phosphate isomerase deficiency is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection and characterized by chronic hemolytic anemia.
Alternative Names: Triosephosphate Isomerase, TIM, Triose-Phosphate Isomerase, TPI1, TPI - Molecular Weight
- 28.8 kDa
- UniProt
- P60174
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