TOLLIP Protein (AA 2-274) (His tag)
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- Target See all TOLLIP Proteins
- TOLLIP (Toll Interacting Protein (TOLLIP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-274
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TOLLIP protein is labelled with His tag.
- Purpose
- Recombinant Human Toll-Interacting Protein/TOLLIP (C-6His)
- Sequence
- ATTVSTQRGP VYIGELPQDF LRITPTQQQR QVQLDAQAAQ QLQYGGAVGT VGRLNITVVQ AKLAKNYGMT RMDPYCRLRL GYAVYETPTA HNGAKNPRWN KVIHCTVPPG VDSFYLEIFD ERAFSMDDRI AWTHITIPES LRQGKVEDKW YSLSGRQGDD KEGMINLVMS YALLPAAMVM PPQPVVLMPT VYQQGVGYVP ITGMPAVCSP GMVPVALPPA AVNAQPRCSE EDLKAIQDMF PNMDQEVIRS VLEAQRGNKD AAINSLLQMG EEPVEHHHHH H
- Characteristics
- Recombinant Human Toll-Interacting Protein is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Pro274) of Human TOLLIP fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product TOLLIP Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5 mM EDTA, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- TOLLIP (Toll Interacting Protein (TOLLIP))
- Alternative Name
- Tollip (TOLLIP Products)
- Synonyms
- IL-1RAcPIP Protein, 4930403G24Rik Protein, 4931428G15Rik Protein, fk22a02 Protein, im:7145391 Protein, wu:fk22a02 Protein, zgc:76985 Protein, TOLLIP Protein, GB17961 Protein, tollip Protein, tollip1 Protein, tollipa Protein, TOLIP Protein, toll interacting protein Protein, toll-interacting protein Protein, toll-interacting protein-like Protein, toll interacting protein L homeolog Protein, toll interacting protein S homeolog Protein, Toll-interleukine I receptor interacting protein I Protein, TOLLIP Protein, Tollip Protein, tollip Protein, LOC552034 Protein, LOC100169858 Protein, tollip.L Protein, tollip.S Protein, LOC100136122 Protein
- Sub Type
- Fusionprotein
- Background
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Toll-Interacting Protein (TOLLIP) is a member of the tollip family. TOLLIP localizes to the cytoplasm. It contains one C2 domain and one CUE domain. TOLLIP is an inhibitory adaptor protein for Toll-like receptors (TLR). The Toll-like receptors pathway is a part of the immune system that recognize structurally conserved molecular patterns of microbial pathogens, resulting in an inflammatory immune response. TOLLIP constitutes a complex with Tom1 to regulate endosomal transferring of ubiquitinated proteins. TOLLIP can negative regulate Toll-like receptors signaling, which may limit the production of proinflammatory mediators during the process of inflammation and infection.
Alternative Names: Toll-Interacting Protein, TOLLIP - Molecular Weight
- 31.3 kDa
- UniProt
- Q9H0E2
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