NMRAL1 Protein (His tag)
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- Target See all NMRAL1 Proteins
- NMRAL1 (NmrA-Like Family Domain Containing 1 (NMRAL1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NMRAL1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product NMRAL1 Protein
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- Comment
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Synonyms: NmrA-like family domain containing 1, HSCARG, SDR48A1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.15 M NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- NMRAL1 (NmrA-Like Family Domain Containing 1 (NMRAL1))
- Alternative Name
- NMRAL1 (NMRAL1 Products)
- Synonyms
- HSCARG Protein, SDR48A1 Protein, 1110025F24Rik Protein, AI256624 Protein, RGD1311451 Protein, NmrA like redox sensor 1 Protein, NmrA-like family domain containing 1 Protein, NMRAL1 Protein, Nmral1 Protein
- Background
- NMRAL1 is a redox sensor protein that undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP+ levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. Recombinant human NMRAL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 35.9kDa(323aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_065728
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