ATG10 Protein (His tag)
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- Target See all ATG10 Proteins
- ATG10 (Autophagy Related 10 (ATG10))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ATG10 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product ATG10 Protein
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Autophagy Related 10 (ATG10) (AA 1-220) protein (His tag)
ATG10 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Autophagy Related 10 (ATG10) (AA 1-220) protein (GST tag)ATG10 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Autophagy Related 10 (ATG10) (AA 1-220) protein (Strep Tag)Crystallography grade ATG10 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Autophagy Related 10 (ATG10) (AA 1-215) protein (Strep Tag)Crystallography grade ATG10 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Autophagy Related 10 (ATG10) (Transcript Variant 3) protein (His tag)ATG10 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Ubiquitin-like-conjugating enzyme ATG10, APG10, APG10L, pp12616
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- ATG10 (Autophagy Related 10 (ATG10))
- Alternative Name
- ATG10 (ATG10 Products)
- Synonyms
- APG10 Protein, APG10L Protein, pp12616 Protein, Atg10 Protein, ATG10 Protein, MGC123341 Protein, im:7153729 Protein, zgc:123341 Protein, ATATG10 Protein, autophagy 10 Protein, DDBDRAFT_0190076 Protein, DDBDRAFT_0252608 Protein, DDB_0190076 Protein, DDB_0252608 Protein, 5330424L23Rik Protein, 5430428K15Rik Protein, AI852123 Protein, Agp10 Protein, Apg10l Protein, Apg10p Protein, Atg10l Protein, autophagy related 10 Protein, autophagy-related 10 Protein, AuTophaGy (yeast Atg homolog) Protein, ATG10 autophagy related 10 homolog (S. cerevisiae) Protein, autophagocytosis-associated family protein Protein, E2-like conjugating enzyme Protein, hypothetical protein Protein, autophagy protein 10 Protein, autophagy-related protein 10 Protein, ATG10 Protein, Atg10 Protein, atg-10 Protein, atg10 Protein, CAALFM_C302930WA Protein, APG10 Protein
- Background
- Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Recombinant human ATG10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 27.7 kDa (243aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_113670
- Pathways
- ER-Nucleus Signaling, Autophagy
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