ATG10 Protein (Transcript Variant 3) (His tag)
Quick Overview for ATG10 Protein (Transcript Variant 3) (His tag) (ABIN2714791)
Target
See all ATG10 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Purification tag / Conjugate
- This ATG10 protein is labelled with His tag.
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Characteristics
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- Recombinant human APG10L / ATG10 (transcript variant 3) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 20 mM Phosphate buffer, 150 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ATG10 (Autophagy Related 10 (ATG10))
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Alternative Name
- Apg10l,atg10
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Background
- Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
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Molecular Weight
- 24.5 kDa
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NCBI Accession
- NP_001124500
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Pathways
- ER-Nucleus Signaling, Autophagy
Target
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