POLR2J3 Protein (His tag)
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- Target See all POLR2J3 Proteins
- POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLR2J3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
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- Comment
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Synonyms: DNA-directed RNA polymerase II subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
- Alternative Name
- POLR2J3 (POLR2J3 Products)
- Synonyms
- POLR2J2 Protein, RPB11b1 Protein, RPB11b2 Protein, RNA polymerase II subunit J3 Protein, POLR2J3 Protein
- Background
- DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 15.5 kDa (138aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001091084
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