GTF2E2 Protein (His tag)
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- Target See all GTF2E2 Proteins
- GTF2E2 (General Transcription Factor IIE, Polypeptide 2 (GTF2E2))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GTF2E2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by using conventional chromatography.
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product GTF2E2 Protein
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General Transcription Factor IIE, Polypeptide 2 (GTF2E2) (AA 1-291) protein (His tag)
GTF2E2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
General Transcription Factor IIE, Polypeptide 2 (GTF2E2) (AA 1-291) protein (GST tag)GTF2E2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
General Transcription Factor IIE, Polypeptide 2 (GTF2E2) (AA 1-291), (full length) protein (His tag)GTF2E2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Comment
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Synonyms: Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-B
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1 M NaCl, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- GTF2E2 (General Transcription Factor IIE, Polypeptide 2 (GTF2E2))
- Alternative Name
- GTF2E2 (GTF2E2 Products)
- Synonyms
- 34kDa Protein, AI462509 Protein, C330006J08Rik Protein, wu:fa05d01 Protein, zgc:77074 Protein, FE Protein, TF2E2 Protein, TFIIE-B Protein, general transcription factor II E, polypeptide 2 (beta subunit) Protein, general transcription factor IIE subunit 2 L homeolog Protein, general transcription factor IIE subunit 2 Protein, general transcription factor IIE, polypeptide 2, beta Protein, Gtf2e2 Protein, gtf2e2.L Protein, gtf2e2 Protein, GTF2E2 Protein
- Background
- GTF2E2, also known as TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57,000 (TFIIE-alpha) and two of 34,000 (TFIIE-beta). TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Recombinant human GTF2E2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 35.6 kDa (315aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_002086
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