HMGB1 Protein (BoxA)

Catalog No. ABIN1176814

Recombinant HMGB1 protein expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for HMGB1 Protein (BoxA) (ABIN1176814)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: Escherichia coli (E. coli)

Application: Cellular Assay (CA), Activation (Act)

Purity: > 98 % It contains no nucleic acids.

Product Details

Protein Characteristics: BoxA

Purpose: BoxA is one of the highly conserved DNA binding domains of HMGB1 protein (HMG boxes), and inhibits HMGB1 effects in vivo.

Sequence: BoxA from HMGB1 consists of 89 amino acid: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF

Characteristics: The BoxA we provide has no difference from the sequence of the human or mouse HMGB1, and as a self-antigen elicits very low immunological responses.
BoxA protects against sepsis in a mouse peritonitis model (Yang et al. 2004), and from hepatitis in a mouse model of HBV infection (Sitia et al. 2007).
Dendritic cells promote their own maturation and T cell proliferation, survival and Th1 differentiation by secreting HMGB1, and BoxA inhibits these activities (Dumitriu et al. 2005).
BoxA also inhibits the chemotactic activity of HMGB1 and the mitogenic activity of HMGB1 on stem cells.
BoxA from HMGB1 is produced in E.coli from an expression plasmid coding for the unmodified mammalian sequence, which is totally identical in human and mouse.

Purification: Purified. The product contains <0.006% v/v of Triton X-114 due to LPS removal procedure.

Endotoxin Level: LPS free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: the lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.

Buffer: 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT. The product contains <0.006% v/v of Triton X-114 due to LPS removal procedure. The remaining traces of Triton X-114 can be removed upon request.

Handling Advice: The product is resistant to repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: 2-8°C. The protein once resuspended can be stored frozen (-20°C). The product is resistant to repeated freezing and thawing.

References

Maroso, Balosso, Ravizza, Liu, Aronica, Iyer, Rossetti, Molteni, Casalgrandi, Manfredi, Bianchi, Vezzani: "Toll-like receptor 4 and high-mobility group box-1 are involved in ictogenesis and can be targeted to reduce seizures." in: Nature medicine, Vol. 16, Issue 4, pp. 413-9, (2010) (PubMed).

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High-mobility group protein B1 (HMGB1)

Molecular Weight: 10.4 kDa

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome