IL12 Protein

Catalog No. ABIN1305066

This Recombinant IL12 protein is expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for IL12 Protein (ABIN1305066)

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Purity: ≥ 95 %

Product Details

Brand: BD Pharmingen™

Specificity: Biological Activity Range: Measured by 2D6 cell proliferation Specific Activity: 0.2 - 2.0 x 10^9 Units /mg
ED50: 5 - 50 pg/ml
Observed dose response: > 200 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation

Characteristics: The recombinant mouse IL-12 was lyophilized in a solution comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, and containing no preservatives. The mass ratio of carrier-protein to mouse IL-12 is 40:1 or 40 µg BSA to 1 µg IL-12. Recombinant mouse IL-12 was found to be > 95% pure by SDS-PAGE and an absorbance assay based on the Beer-Lambert law.

Purification: Recombinant mouse IL-12 p70 is provided lyophilized, containing bovine serum albumin and no preservatives. Recombinant mouse IL-12 p70 was found to be ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beer-Lambert law.

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of mouse IL-12, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: This mouse IL-12 is useful as a quantitative standard for measuring mouse IL-12 protein levels in an IL-12 specific sandwich ELISA with the purified 9A5 antibody (Cat. No. 554658) as a capture antibody and the biotinylated C17.8 (Cat. No. 554476) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-12 standard from 4000-30 pg/ml should be included in each ELISA plate.

Comment:

BD Pharmingen™ Recombinant Mouse IL-12 p70 - Purified - Clone NA - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized in an aqueous buffered solution containing BSA.

Handling Advice: Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Schoenhaut, Chua, Wolitzky, Quinn, Dwyer, McComas, Familletti, Gately, Gubler: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 (p70)

Background: Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome