Interferon gamma Protein (IFNG)

Catalog No. ABIN1305089

Product Details

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Biological Activity Activity Test: Biological activity is measured in an anti-viral bioassay using L929 indicator cells
Specific Activity Range: 0.1-1.0x10^8Unit /mg
ED50: 0.1 - 1.0 ng/ml
Observed dose response relationship: 100 fold.
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Characteristics: Recombinant rat IFN-gamma is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, and containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat IFN-gamma is > 95% pure, as determined by SDS-PAGE analysis and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant rat IFN-γ is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant rat IFN-γ is ≥ 95 % pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng/µg of recombinant protein, as measured in a chromogenic LAL assay.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD Pharmingen™ Recombinant Rat IFN-γ - Reactivity Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Handling Advice: Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences involve the action of IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Farrar, Schreiber: "The molecular cell biology of interferon-gamma and its receptor." in: Annual review of immunology, Vol. 11, pp. 571-611, (1993) (PubMed).

Gray, Goeddel: "Cloning and expression of murine immune interferon cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 19, pp. 5842-6, (1983) (PubMed).

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma (IFNG Products)

Synonyms: IFG Protein, IFI Protein, IFN-g Protein, Ifg Protein, IFNG2 Protein, IFN-gamma Protein, IFN-G Protein, IFNG Protein, IFNgamma Protein, TCRalpha Protein, INF-G Protein, ifng Protein, interferon gamma Protein, interferon, gamma 1-2 Protein, IFNG Protein, Ifng Protein, ifng1-2 Protein

Background: Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. In addition, IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences involve the action of IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy