IL-6 Protein

Catalog No. ABIN1305109

Product Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Specificity: Biological Activity: Activity Tested by proliferation assay using NFS-60 indicator cells
Specific Activity: 1.3 - 6.7 x 10^7 Units /mg
ED50: 150-750 pg/ml
Observed dose response: 64 fold.
A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation

Characteristics: Recombinant mouse IL-6 protein is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered Dulbecco’s PBS (pH 7.2) containing 1.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, and glycerol, with no preservatives. The recombinant mouse IL-6 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant mouse IL-6 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IL-6 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of mouse IL-6, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: The recombinant mouse IL-6 is useful as a quantitative standard for measuring mouse IL-6 protein levels in an IL-6 specific sandwich ELISA with the purified MP5-20F3 antibody (Cat. No. 554400) as a capture antibody and the biotinylated MP5-32C11 (Cat. No. 554402) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-6 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Ligand Blocking Control for Immunofluorescent Staining and Flow Cytometric Analysis: The recombinant mouse IL-6 can be used as a blocking control to demonstrate specificity of IL-6 staining (for instance staining with the PE-MP5-20F3 antibody, Cat. No. 554401). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

Comment:

BD Pharmingen™ Recombinant Mouse IL-6 - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interleukin-6 (IL-6), also known as IFN-β2, BSF-2, and HPGF, is a multifunctional cytokine which regulates immune responses, acute-phase reactions and hematopoiesis. Reported cellular targets for IL-6 action include hepatocytes, B and T lymphocytes, neurons, tumor cells and multipotent hematopoietic cells. Mouse IL-6 is a 21.7 kD protein containing 187 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Chiu, Moulds, Coffman, Rennick, Lee: "Multiple biological activities are expressed by a mouse interleukin 6 cDNA clone isolated from bone marrow stromal cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 19, pp. 7099-103, (1988) (PubMed).

Van Snick, Cayphas, Szikora, Renauld, Van Roost, Boon, Simpson: "cDNA cloning of murine interleukin-HP1: homology with human interleukin 6." in: European journal of immunology, Vol. 18, Issue 2, pp. 193-7, (1988) (PubMed).

Van Snick, Cayphas, Vink, Uyttenhove, Coulie, Rubira, Simpson: "Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 24, pp. 9679-83, (1987) (PubMed).

Weinstein, Ihle, Lavu, Reddy: "Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5010-4, (1986) (PubMed).

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6 (IL6 Products)

Synonyms: BSF2 Protein, HGF Protein, HSF Protein, IFNB2 Protein, IL-6 Protein, Il-6 Protein, ILg6 Protein, Ifnb2 Protein, il6 Protein, CHIL-6 Protein, interleukin 6 Protein, interleukin-6 Protein, IL6 Protein, Il6 Protein, il-6 Protein, IL-6 Protein

Background: Interleukin-6 (IL-6), also known as IFN-β2, BSF-2, and HPGF, is a multifunctional cytokine which regulates immune responses, acute-phase reactions and hematopoiesis. Reported cellular targets for IL-6 action include hepatocytes, B and T lymphocytes, neurons, tumor cells and multipotent hematopoietic cells. Mouse IL-6 is a 21.7 kD protein containing 187 amino acid residues.

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome