IL-2 Protein

Catalog No. ABIN1305117

Product Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Specificity: Biological Activity: Activity Range is measured in a proliferation assay using CTLL-2 indicator cells
Specific Activity: 0.1 - 1.0 x 10^9 Units/mg
ED50: 10 - 100 pg/ml
Observed dose response relationship: 80 fold.
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.

Characteristics: Recombinant mouse IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing 2.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-2 is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant mouse IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution and bovine serum albumin, with no preservatives. Recombinant mouse IL-2 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per μg of mouse IL-2, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant mouse IL-2 is useful as a quantitative standard for measuring mouse IL-2 protein levels in an IL-2 specific sandwich ELISA with the purified JES6-1A12 antibody (Cat. No. 554424) as a capture antibody and the biotinylated JES6-5H4 antibody (Cat.No. 554426) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-2 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IL-2 in serum or plasma the mouse IL-2 BD OptEIA™ Set (Cat. No. 555148) is specially formulated and recommended.

Comment:

BD Pharmingen™ Recombinant Mouse IL-2 - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Preservative: Azide free

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Kashima, Nishi-Takaoka, Fujita, Taki, Yamada, Hamuro, Taniguchi: "Unique structure of murine interleukin-2 as deduced from cloned cDNAs." in: Nature, Vol. 313, Issue 6001, pp. 402-4, (1985) (PubMed).

Mochizuki, Watson, Gillis: "Biochemical separation of interleukin 2." in: Journal of immunological methods, Vol. 39, Issue 3, pp. 185-201, (1981) (PubMed).

Watson, Mochizuki: "Interleukin 2: a class of T cell growth factors." in: Immunological reviews, Vol. 51, pp. 257-78, (1981) (PubMed).

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2 (IL2 Products)

Synonyms: IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein

Background: Interleukin-2 (IL-2) is a cytokine which was first described and characterized based upon its potent ability to modulate lymphocyte reactivity and promote long term in vitro culture of antigen-specific effector T lymphocytes. It is responsible for the activities previously ascribed to T cell growth factor, thymocyte stimulating factor, helper-factor and T cell replacing factor, to name a few. IL-2 has also been reported to act on neuronal cells. Mouse IL-2 is a 17.2 kD protein containing 149 amino acid residues.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation