GRP78 Protein (AA 19-654) (GST tag)

Catalog No. ABIN1307202

Product Details

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Protein Type: Recombinant

Protein Characteristics: AA 19-654

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This GRP78 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: HSPA5 (Human) Recombinant Protein (P01)

Sequence: EEEDKKEDVG TVVGIDLGTT YSCVGVFKNG RVEIIANDQG NRITPSYVAF TPEGERLIGD AAKNQLTSNP ENTVFDAKRL IGRTWNDPSV QQDIKFLPFK VVEKKTKPYI QVDIGGGQTK TFAPEEISAM VLTKMKETAE AYLGKKVTHA VVTVPAYFND AQRQATKDAG TIAGLNVMRI INEPTAAAIA YGLDKREGEK NILVFDLGGG TFDVSLLTID NGVFEVVATN GDTHLGGEDF DQRVMEHFIK LYKKKTGKDV RKDNRAVQKL RREVEKAKRA LSSQHQARIE IESFYEGEDF SETLTRAKFE ELNMDLFRST MKPVQKVLED SDLKKSDIDE IVLVGGSTRI PKIQQLVKEF FNGKEPSRGI NPDEAVAYGA AVQAGVLSGD QDTGDLVLLD VCPLTLGIET VGGVMTKLIP RNTVVPTKKS QIFSTASDNQ PTVTIKVYEG ERPLTKDNHL LGTFDLTGIP PAPRGVPQIE VTFEIDVNGI LRVTAEDKGT GNKNKITITN DQNRLTPEEI ERMVNDAEKF AEEDKKLKER IDTRNELESY AYSLKNQIGD KEKLGGKLSS EDKETMEKAV EEKIEWLESH QDADIEDFKA KKKELEEIVQ PIISKLYGSA GPPPTGEEDT AEKDEL

Characteristics: Human HSPA5 full-length ORF ( AAH20235.1, 19 a.a. - 654 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Taylor, Gercel-Taylor, Parker: "Patient-derived tumor-reactive antibodies as diagnostic markers for ovarian cancer." in: Gynecologic oncology, Vol. 115, Issue 1, pp. 112-20, (2009) (PubMed).

Target Details

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5 (HSPA5 Products)

Synonyms: GRP78 Protein, BiP Protein, GRP-78 Protein, grp78 Protein, hspa5a Protein, BIP Protein, MIF2 Protein, AL022860 Protein, AU019543 Protein, Bip Protein, D2Wsu141e Protein, D2Wsu17e Protein, Grp78 Protein, Hsce70 Protein, SEZ-7 Protein, Sez7 Protein, baffled Protein, mBiP Protein, cb865 Protein, fb60h09 Protein, fi36d04 Protein, wu:fb60h09 Protein, wu:fi36d04 Protein, zgc:55994 Protein, zgc:77606 Protein, 78 kDa glucose-regulated protein Protein, heat shock protein family A (Hsp70) member 5 Protein, BiP/GRP78 Protein, glucose-regulated protein 78 Protein, putative glucose-regulated protein 78 Protein, Hsp70 family ATPase KAR2 Protein, heat shock protein family A (Hsp70) member 5 S homeolog Protein, heat shock 70 kDa protein 5a Protein, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) Protein, heat shock protein 5 Protein, heat shock protein family A member 5 Protein, CpipJ_CPIJ003550 Protein, HSPA5 Protein, grp78 Protein, LOC100533358 Protein, BiP/grp78 Protein, Tc00.1047053506585.40 Protein, Tb11.02.5450 Protein, Tb11.02.5500 Protein, LMJF_28_1200 Protein, KAR2 Protein, LOC100135840 Protein, hspa5.S Protein, hspa5 Protein, Hspa5 Protein

Background: Full Gene Name: heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa)
Synonyms: BIP,FLJ26106,GRP78,MIF2

Gene ID: 3309

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling