POLA2 Protein (AA 1-598) (GST tag)
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- Target See all POLA2 Proteins
- POLA2 (Polymerase (DNA Directed), alpha 2 (70kD Subunit) (POLA2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-598
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This POLA2 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- POLA2 (Human) Recombinant Protein (P01)
- Sequence
- MSASAQQLAE ELQIFGLDCE EALIEKLVEL CVQYGQNEEG MVGELIAFCT STHKVGLTSE ILNSFEHEFL SKRLSKARHS TCKDSGHAGA RDIVSIQELI EVEEEEEILL NSYTTPSKGS QKRAISTPET PLTKRSVSTR SPHQLLSPSS FSPSATPSQK YNSRSNRGEV VTSFGLAQGV SWSGRGGAGN ISLKVLGCPE ALTGSYKSMF QKLPDIREVL TCKIEELGSE LKEHYKIEAF TPLLAPAQEP VTLLGQIGCD SNGKLNNKSV ILEGDREHSS GAQIPVDLSE LKEYSLFPGQ VVIMEGINTT GRKLVATKLY EGVPLPFYQP TEEDADFEQS MVLVACGPYT TSDSITYDPL LDLIAVINHD RPDVCILFGP FLDAKHEQVE NCLLTSPFED IFKQCLRTII EGTRSSGSHL VFVPSLRDVH HEPVYPQPPF SYSDLSREDK KQVQFVSEPC SLSINGVIFG LTSTDLLFHL GAEEISSSSG TSDRFSRILK HILTQRSYYP LYPPQEDMAI DYESFYVYAQ LPVTPDVLII PSELRYFVKD VLGCVCVNPG RLTKGQVGGT FARLYLRRPA ADGAERQSPC IAVQVVRI
- Characteristics
- Human POLA2 full-length ORF ( AAH01347, 1 a.a. - 598 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Polymerase (DNA Directed), alpha 2 (70kD Subunit) (POLA2) (AA 1-598) protein (Strep Tag)
Crystallography grade POLA2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Polymerase (DNA Directed), alpha 2 (70kD Subunit) (POLA2) (AA 1-600) protein (Strep Tag)Crystallography grade POLA2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- POLA2 (Polymerase (DNA Directed), alpha 2 (70kD Subunit) (POLA2))
- Alternative Name
- POLA2 (POLA2 Products)
- Synonyms
- DNA polymerase alpha 2 Protein, F12B7.19 Protein, F12B7_19 Protein, zgc:56124 Protein, zgc:77841 Protein, wu:fb48f05 Protein, AI573378 Protein, DNA polymerase alpha 2, accessory subunit Protein, DNA polymerase alpha 2 Protein, polymerase (DNA directed), alpha 2 Protein, polymerase (DNA directed), alpha 2, accessory subunit L homeolog Protein, polymerase (DNA directed), alpha 2, accessory subunit Protein, POLA2 Protein, pola2 Protein, pola2.L Protein, Pola2 Protein
- Background
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Full Gene Name: polymerase (DNA directed), alpha 2 (70kD subunit)
Synonyms: FLJ21662,FLJ37250 - Gene ID
- 23649
- Pathways
- Telomere Maintenance, Mitotic G1-G1/S Phases, DNA Replication, Protein targeting to Nucleus, Synthesis of DNA, SARS-CoV-2 Protein Interactome
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