POLE Protein (AA 1-370) (GST tag)
-
- Target See all POLE products
- POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-370
- Origin
- Human
- Source
- Wheat germ
- Purification tag / Conjugate
- This POLE protein is labelled with GST tag.
- Application
- Affinity Purification (AP), Antibody Array (AA), ELISA, Western Blotting (WB)
- Purpose
- POLE (Human) Recombinant Protein (P01)
- Sequence
- MDPSNYGGIK GKVSSRIHCG LQDSQKAGGA EDEQENEDDE EERDGEEEEE AEESNVEDLL ENNWNILQFL PQAASCQNYF LMIVSAYIVA VYHCMKDGLR RSAPGSTPVR RRGASQLSQE AEGAVGALPG MITFSQDYVA NELTQSFFTI TQKIQKKVTG SRNSTELSEM FPVLPGSHLL LNNPALEFIK YVCKVLSLDT NITNQVNKLN RDLLRLVDVG EFSEEAQFRD PCRSYVLPEV ICRSCNFCRD LDLCKDSSFS EDGAVLPQWL CSNCQAPYDS SAIEMTLVEV LQKKLVAFTL QDLVCLKCRG VKETSMPVYC SCAGDFALTI HTQVFMEQIG IFRNIAQHYG MSYLLETLEW LLQKNPQLGH
- Characteristics
- Human POLE full-length ORF ( AAH21559, 1 a.a. - 370 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
-
- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
-
- Target
- POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))
- Alternative Name
- POLE (POLE Products)
- Synonyms
- CRCS12 Protein, FILS Protein, POLE1 Protein, DPE2 Protein, DNA polymerase epsilon, catalytic subunit Protein, polymerase (DNA directed), epsilon Protein, DNA polymerase epsilon 2, accessory subunit Protein, polymerase (DNA directed), epsilon 2 (p59 subunit) Protein, POLE Protein, Pole Protein, POLE2 Protein, Pole2 Protein
- Background
-
Full Gene Name: polymerase (DNA directed), epsilon
Synonyms: DKFZp434F222,FLJ21434,POLE1 - Gene ID
- 5426
- Pathways
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
-