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POLE Protein (AA 1-370) (GST tag)

POLE Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Catalog No. ABIN1315634
  • Target See all POLE products
    POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-370
    Origin
    Human
    Source
    Wheat germ
    Purification tag / Conjugate
    This POLE protein is labelled with GST tag.
    Application
    Affinity Purification (AP), Antibody Array (AA), ELISA, Western Blotting (WB)
    Purpose
    POLE (Human) Recombinant Protein (P01)
    Sequence
    MDPSNYGGIK GKVSSRIHCG LQDSQKAGGA EDEQENEDDE EERDGEEEEE AEESNVEDLL ENNWNILQFL PQAASCQNYF LMIVSAYIVA VYHCMKDGLR RSAPGSTPVR RRGASQLSQE AEGAVGALPG MITFSQDYVA NELTQSFFTI TQKIQKKVTG SRNSTELSEM FPVLPGSHLL LNNPALEFIK YVCKVLSLDT NITNQVNKLN RDLLRLVDVG EFSEEAQFRD PCRSYVLPEV ICRSCNFCRD LDLCKDSSFS EDGAVLPQWL CSNCQAPYDS SAIEMTLVEV LQKKLVAFTL QDLVCLKCRG VKETSMPVYC SCAGDFALTI HTQVFMEQIG IFRNIAQHYG MSYLLETLEW LLQKNPQLGH
    Characteristics
    Human POLE full-length ORF ( AAH21559, 1 a.a. - 370 a.a.) recombinant protein with GST-tag at N-terminal.
    Purification
    in vitro wheat germ expression system
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Preparation method: in vitro, wheat germ expression system
    Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

    Restrictions
    For Research Use only
  • Buffer
    50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
    Handling Advice
    Aliquot to avoid repeated freezing and thawing.
    Storage
    -80 °C
    Storage Comment
    Best use within three months from the date of receipt of this protein.
  • Target
    POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit (POLE))
    Alternative Name
    POLE (POLE Products)
    Synonyms
    CRCS12 Protein, FILS Protein, POLE1 Protein, DPE2 Protein, DNA polymerase epsilon, catalytic subunit Protein, polymerase (DNA directed), epsilon Protein, DNA polymerase epsilon 2, accessory subunit Protein, polymerase (DNA directed), epsilon 2 (p59 subunit) Protein, POLE Protein, Pole Protein, POLE2 Protein, Pole2 Protein
    Background
    Full Gene Name: polymerase (DNA directed), epsilon
    Synonyms: DKFZp434F222,FLJ21434,POLE1
    Gene ID
    5426
    Pathways
    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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