T-Bet Protein (AA 400-535) (GST tag)
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- Target See all T-Bet Proteins
- T-Bet
- Protein Type
- Recombinant
- Protein Characteristics
- AA 400-535
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This T-Bet protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- TBX21 (Human) Recombinant Protein (Q01)
- Sequence
- RAVSMKPAFL PSAPGPTMSY YRGQEVLAPG AGWPVAPQYP PKMGPASWFR PMRTLPMEPG PGGSEGRGPE DQGPPLVWTE IAPIRPESSD SGLGEGDSKR RRVSPYPSSG DSSSPAGAPS PFDKEAEGQF YNYFPN
- Characteristics
- Human TBX21 partial ORF (NP_037483, 400 a.a. - 535 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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T-Bet (AA 387-486) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
T-Bet (AA 2-535) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
T-Bet (AA 1-535) protein (GST tag)Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
T-Bet (AA 1-535) protein (Strep Tag)Crystallography grade Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
T-Bet (AA 1-530) protein (Strep Tag)Crystallography grade Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
T-Bet protein (Myc-DYKDDDDK Tag)Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- T-Bet
- Alternative Name
- TBX21 (T-Bet Products)
- Synonyms
- T-PET Protein, T-bet Protein, TBET Protein, TBLYM Protein, TBT1 Protein, Tbet Protein, Tblym Protein, T-box 21 Protein, TBX21 Protein, Tbx21 Protein
- Background
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Full Gene Name: T-box 21
Synonyms: T-PET,T-bet,TBET,TBLYM - Gene ID
- 30009
- NCBI Accession
- NM_013351
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