Vimentin Protein (VIM) (full length) (rho-1D4 tag)

Catalog No. ABIN7126035

Product Details

Target: Vimentin (VIM)

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This Vimentin protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)

Sequence: PRHLEPAGSN RSYVTTSTRT YSLGALRPST SRSLYSSSPG GAYVTRSSAV RLRSSMPGVR LLQDSVDFSL ADAINTEFKN TRTNEKVELQ ELNDRFADYI DKVRFLEQQN KILLAELEQL KGQGKSRLGD LYEEEMRELR RQVDQLTNDK ARVEVERDNL AEDIIRLREK LQEEMLQREE AESTLQSFRQ DVDNASLARL DLERKVESLQ EEIAFLKKLH DEEIQELQAQ IQEQHVQIDV DVSKPDLTAA LRDVRQQYES VAAKNLQEAE EWYKSKFADL SEAANRNNDA LRQAKQESNE YRRQVQSLTC EVDALKGTNE SLERQMREME ENFALEAANY QDTIGRLQDE IQNMKEEMAR HLREYQDLLN VKMALDIEIA TYRKLLEGEE SRISLPLPNF SSLNLRETNL ESLPLVDTHS KRTLLIKTVE TRDGQVINET SQHHDDLE
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Vimentin (Fragment) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Vimentin (VIM)

Alternative Name: Vimentin (VIM Products)

Synonyms: CTRCT30 Protein, cb28 Protein, vime Protein, vim Protein, vim1 Protein, vim2 Protein, VIM Protein, Vimentin Protein, vim4 Protein, vimentin Protein, vimentin L homeolog Protein, vimentin S homeolog Protein, VIM Protein, Vim Protein, vim Protein, vim.L Protein, vim.S Protein

Background: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally., Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. {ECO:0000250}.

UniProt: P48670

Pathways: Caspase Cascade in Apoptosis