IL-1 beta Protein (AA 117-269)

Catalog No. ABIN1343986

Recombinant IL-1 beta protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-1 beta Protein (AA 117-269) (ABIN1343986)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 117-269

Purpose: IL-1beta (human) (rec.) (untagged)

Specificity: Binds to human IL-1R.

Cross-Reactivity: Human

Characteristics: Human IL-1beta (aa 117-269) is untagged.

Endotoxin Level: <0.1EU/μg purified protein (LAL test).

Biological Activity Comment: Activates the NF-kappaB with an ED50 of <5ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1beta

Background:

Interleukin-1beta, IL-1F2

IL-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1beta belongs to the IL-1 family of proteins that are involved in the inflammatory response. IL-1beta in most situations requires the inflammasome complex to be cleaved and secreted.

Molecular Weight: ~17kDa (SDS-PAGE)

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome