IL-1 beta Protein (AA 117-269) (His-Avi Tag,Biotin)

Catalog No. ABIN7274883

Product Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Protein Characteristics: AA 117-269

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This IL-1 beta protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human IL-1 Beta/IL-1F2 Protein

Sequence: Ala117-Ser269

Characteristics: Recombinant Biotinylated Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-Terminus.It contains Ala117-Ser269.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-1R2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1 Beta (IL1B Products)

Synonyms: IL-1 Protein, IL1-BETA Protein, IL1F2 Protein, IL-1BETA Protein, IL1beta Protein, il1-b Protein, zgc:111873 Protein, IL-1B Protein, IL-1beta Protein, Il-1b Protein, IL1B Protein, IL-1 beta Protein, IL-1b Protein, interleukin 1 beta Protein, interleukin 1, beta Protein, IL1B Protein, il1b Protein, Il1b Protein

Background: Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.

Molecular Weight: 20.1 kDa same as Tris-Bis PAGE result.

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome