IL-2 Superkine (AA 21-153), (Extracellular Domain) protein (Fc Tag)
Origin: Human
Host: HEK-293 Cells
Recombinant
>95 % (SDS-PAGE)
SDS
1 image
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- Target
- IL-2 Superkine
- Protein Type
- Recombinant
- Protein Characteristics
- Extracellular Domain, AA 21-153
- Origin
- Human
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- Fc Tag
- Application
- SDS-PAGE (SDS)
- Specificity
- Binds to human and mouse IL-2R.
- Cross-Reactivity
- Human
- Characteristics
- The extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) is fused at the C-terminus to the Fc portion of human IgG2.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg protein (LAL test, Lonza).
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. - Expiry Date
- 12 months
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- Target
- IL-2 Superkine
- Background
- Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-gamma and TNF-alpha and -beta. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens. IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Ralpha (CD25), IL-2Rbeta and IL-2Rgamma. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rbeta and IL-2Rgamma. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rbeta and IL-2Rgamma receptors. IL-2 Superkine (Fc) is an artificial variant of IL-2 containing mutations at positions L80F / R81D / L85V / I 86V / I92F. These mutations are located in the molecule's core that acts to stabilize the structure and to give it a receptor-binding conformation mimicking native IL-2 bound to CD25. These mutations effectively eliminate the functional requirement of IL-2 for CD25 expression and elicit proliferation of T cells. Compared to IL-2, the IL-2 superkine induces superior expansion of cytotoxic T cells, leading to improved antitumour responses in vivo, and elicits proportionally less toxicity by lowering the expansion of Tregulatory cells and reducing pulmonary oedema.
- Molecular Weight
- 42kDa
- UniProt
- P60568
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