LAG3 Protein (Fc Tag)

Catalog No. ABIN1344460

Product Details

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: CHO Cells

Purification tag / Conjugate: This LAG3 protein is labelled with Fc Tag.

Application: SDS-PAGE (SDS), Flow Cytometry (FACS)

Purpose: LAG-3 (mouse):Fc (mouse) (rec.)

Specificity: Binds to human and mouse MHC class II.

Cross-Reactivity: Human, Mouse

Characteristics: The sequence coding for the 4 extracellular Ig-like domains of mouse LAG-3 (D1-D4) is fused to the Fc portion of mouse IgG2a (hinge-CH2-CH3).

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Induces maturation of mouse dendritic cells in vitro. Induces CD4 and CD8 antigen-specific T cells responses when coinjected s.c. in mice, together with the antigen.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: In PBS.

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: -20 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Cappello, Triebel, Iezzi, Caorsi, Quaglino, Lollini, Amici, Di Carlo, Musiani, Giovarelli, Forni: "LAG-3 enables DNA vaccination to persistently prevent mammary carcinogenesis in HER-2/neu transgenic BALB/c mice." in: Cancer research, Vol. 63, Issue 10, pp. 2518-25, (2003) (PubMed).

Target Details

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG-3 (LAG3 Products)

Background:

Lymphocyte Activation Gene-3, FDC Protein, CD223

Lymphocyte activation gene 3 (LAG-3, CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.

Molecular Weight: ~180kDa (SPS-PAGE, nonreducing), ~80kDa (SPS-PAGE, reducing)

UniProt: Q61790

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints