APAF1 Protein (AA 1138-1237) (GST tag)
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- Target See all APAF1 Proteins
- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1138-1237
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This APAF1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- APAF1 (Human) Recombinant Protein (Q01)
- Sequence
- GEIRIWNVSN GELLHLCAPL SEEGAATHGG WVTDLCFSPD GKMLISAGGY IKWWNVVTGE SSQTFYTNGT NLKKIHVSPD FKTYVTVDNL GILYILQTLE
- Characteristics
- Human APAF1 partial ORF ( NP_037361, 1138 a.a. - 1237 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1-1248) protein (Strep Tag)
Crystallography grade APAF1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1-1249) protein (Strep Tag)Crystallography grade APAF1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Apoptotic Peptidase Activating Factor 1 (APAF1) proteinAPAF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 104-415) protein (His tag)APAF1 Origin: Human Host: Yeast Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
- Alternative Name
- APAF1 (APAF1 Products)
- Synonyms
- APAF1 Protein, ARK Protein, Apaf-1 Protein, Apaf1 Protein, CG6829 Protein, D-Apaf-1 Protein, DARK Protein, Dapaf-1 Protein, Dapaf-1/HAC-1 Protein, Dark Protein, Dark/Apaf-I Protein, Dark/Dapaf-1/HAC1 Protein, Dark/Hac-1/dApaf-1 Protein, Dark/Hac-1/dApaf1 Protein, Dmel\\CG6829 Protein, Hac-1 Protein, Hac-1/Dark Protein, Hac1 Protein, T1 Protein, anon-53Fa Protein, apaf-1 Protein, apaf1 Protein, arc Protein, ark Protein, dAPAF-1 Protein, dApaf-1 Protein, dApaf-1/DARK/HAC-1 Protein, dApaf1 Protein, dArk Protein, dapaf Protein, dapaf-1 Protein, dapaf-1L Protein, dapaf-1S Protein, dark Protein, dark/dapaf-1/hac-1 Protein, dark/hac-1/dapaf-1 Protein, hac-1 Protein, hac1 Protein, l(2)SH0173 Protein, APAF-1 Protein, CED4 Protein, 6230400I06Rik Protein, Apaf1l Protein, fog Protein, mKIAA0413 Protein, zgc:136543 Protein, Death-associated APAF1-related killer Protein, apoptotic peptidase activating factor 1 L homeolog Protein, apoptotic peptidase activating factor 1 Protein, Dark Protein, apaf1.L Protein, apaf1 Protein, APAF1 Protein, Apaf1 Protein
- Background
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Full Gene Name: apoptotic peptidase activating factor 1
Synonyms: CED4,DKFZp781B1145 - Gene ID
- 317
- NCBI Accession
- NM_013229
- Pathways
- p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
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