BDNF Protein (AA 1-255) (GST tag)

Catalog No. ABIN1346621

This Recombinant BDNF protein is produced in Wheat germ. There is 1 publication available.

Quick Overview for BDNF Protein (AA 1-255) (GST tag) (ABIN1346621)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-255

Purification tag / Conjugate: This BDNF protein is labelled with GST tag.

Purpose: BDNF (Human) Recombinant Protein (P02)

Sequence: MFHQVRRVMT ILFLTMVISY FGCMKAAPMK EANIRGQGGL AYPGVRTHGT LESVNGPKAG SRGLTSLADT FEHVIEELLD EDQKVRPNEE NNKDADLYTS RVMLSSQVPL EPPLLFLLEE YKNYLDAANM SMRVRRHSDP ARRGELSVCD SISEWVTAAD KKTAVDMSGG TVTVLEKVPV SKGQLKQYFY ETKCNPMGYT KEGCRGIDKR HWNSQCRTTQ SYVRALTMDS KKRIGWRFIR IDTSCVCTLT IKRGR

Characteristics: Human BDNF full-length ORF ( NP_733927.1, 1 a.a. - 255 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Martin Bauknight, Chakrabarty, Hwang, Malone, Joshi, Bruce, Sander Connolly, Winfree, Cunningham, Martin, Haque: "Convection enhanced drug delivery of BDNF through a microcannula in a rodent model to strengthen connectivity of a peripheral motor nerve bridge model to bypass spinal cord injury." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 19, Issue 4, pp. 563-9, (2012) (PubMed).

Target Details

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: Full Gene Name: brain-derived neurotrophic factor
Synonyms: MGC34632

Gene ID: 627

NCBI Accession: NM_170731

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity