CD40 Ligand Protein (CD40LG) (Trimer) (His-DYKDDDDK Tag,Biotin)
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- Target See all CD40 Ligand (CD40LG) Proteins
- CD40 Ligand (CD40LG)
- Protein Type
- Recombinant
- Protein Characteristics
- Trimer
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD40 Ligand protein is labelled with His-DYKDDDDK Tag,Biotin.
- Sequence
- Met113-Leu261
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.77μg/ml determined by ELISA. See testing image for detail.
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- Discover our top product CD40LG Protein
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CD40 Ligand (CD40LG) (Trimer) protein (Fc Tag)
CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (Trimer) protein (His-DYKDDDDK Tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (Trimer) protein (Fc Tag)CD40LG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD40 Ligand (CD40LG) (AA 116-261) protein (His-DYKDDDDK-Strep II Tag)CD40LG Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG Origin: Human, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (Active) protein (His tag)CD40LG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
CD40 Ligand (CD40LG) proteinCD40LG Origin: Human Host: Human Cells Recombinant >90 % as determined by reducing SDS-PAGE.
CD40 Ligand (CD40LG) (AA 112-260) protein (His tag)CD40LG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
CD40 Ligand (CD40LG) (AA 1-261) protein (GST tag)CD40LG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
- Storage
- -80 °C
- Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD40 Ligand (CD40LG)
- Alternative Name
- CD40 Ligand (CD40LG Products)
- Synonyms
- CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein
- Background
- CD40L, CD40LG, CD154, TNFSF5, TNFSF5IMD3, CD40LIGM, gp39, HIGM1, T-BAM, TRAP, IGM, IMD3, CD40 Ligand,CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
- Molecular Weight
- 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
- UniProt
- P29965
- Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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