CRBN Protein (AA 1-442) (GST tag)
(1 reference)-
- Target See all CRBN Proteins
- CRBN (Cereblon (CRBN))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-442
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This CRBN protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- CRBN (Human) Recombinant Protein (P01)
- Sequence
- MAGEGDQQDA AHNMGNHLPL LPAESEEEDE MEVEDQDSKE AKKPNIINFD TSLPTSHTYL GADMEEFHGR TLHDDDSCQV IPVLPQVMMI LIPGQTLPLQ LFHPQEVSMV RNLIQKDRTF AVLAYSNVQE REAQFGTTAE IYAYREEQDF GIEIVKVKAI GRQRFKVLEL RTQSDGIQQA KVQILPECVL PSTMSAVQLE SLNKCQIFPS KPVSREDQCS YKWWQKYQKR KFHCANLTSW PRWLYSLYDA ETLMDRIKKQ LREWDENLKD DSLPSNPIDF SYRVAACLPI DDVLRIQLLK IGSAIQRLRC ELDIMNKCTS LCCKQCQETE ITTKNEIFSL SLCGPMAAYV NPHGYVHETL TVYKACNLNL IGRPSTEHSW FPGYAWTVAQ CKICASHIGW KFTATKKDMS PQKFWGLTRS ALLPTIPDTE DEISPDKVIL CL
- Characteristics
- Human CRBN full-length ORF ( NP_057386.2, 1 a.a. - 442 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Cereblon (CRBN) (AA 1-80) protein (His-IF2DI Tag)
CRBN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cereblon (CRBN) (AA 1-442) protein (His-IF2DI Tag)CRBN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cereblon (CRBN) (AA 343-442) protein (GST tag)CRBN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cereblon (CRBN) (AA 1-445) protein (Strep Tag)Crystallography grade CRBN Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Cereblon (CRBN) (AA 1-442) protein (Strep Tag)Crystallography grade CRBN Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Identification of salt-inducible kinase 3 as a novel tumor antigen associated with tumorigenesis of ovarian cancer." in: Oncogene, Vol. 30, Issue 33, pp. 3570-84, (2011) (PubMed).
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: "Identification of salt-inducible kinase 3 as a novel tumor antigen associated with tumorigenesis of ovarian cancer." in: Oncogene, Vol. 30, Issue 33, pp. 3570-84, (2011) (PubMed).
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- Target
- CRBN (Cereblon (CRBN))
- Alternative Name
- CRBN (CRBN Products)
- Synonyms
- zgc:92404 Protein, mrt2a Protein, F3N11.21 Protein, MRT2 Protein, MRT2A Protein, 2610203G15Rik Protein, 2900045O07Rik Protein, AF229032 Protein, AW108261 Protein, piL Protein, cereblon Protein, ATP-dependent protease La (LON) domain protein Protein, crbn Protein, CRBN Protein, AT2G25740 Protein, Crbn Protein
- Background
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Full Gene Name: cereblon
Synonyms: DKFZp781K0715,MGC27358,MRT2A - Gene ID
- 51185
- NCBI Accession
- NM_016302
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