Doublecortin Protein (DCX) (AA 1-360) (GST tag)
(1 reference)-
- Target See all Doublecortin (DCX) Proteins
- Doublecortin (DCX)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-360
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Doublecortin protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- DCX (Human) Recombinant Protein (P01)
- Sequence
- MELDFGHFDE RDKTSRNMRG SRMNGLPSPT HSAHCSFYRT RTLQALSNEK KAKKVRFYRN GDRYFKGIVY AVSSDRFRSF DALLADLTRS LSDNINLPQG VRYIYTIDGS RKIGSMDELE EGESYVCSSD NFFKKVEYTK NVNPNWSVNV KTSANMKAPQ SLASSNSAQA RENKDFVRPK LVTIIRSGVK PRKAVRVLLN KKTAHSFEQV LTDITEAIKL ETGVVKKLYT LDGKQVTCLH DFFGDDDVFI ACGPEKFRYA QDDFSLDENE CRVMKGNPSA TAGPKASPTP QKTSAKSPGP MRRSKSPADS ANGTSSSQLS TPKSKQSPIS TPTSPGSLRK HKDLYLPLSL DDSDSLGDSM
- Characteristics
- Human DCX full-length ORF ( AAH27925, 1 a.a. - 360 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Doublecortin (DCX) (AA 107-208) protein (His-IF2DI Tag)
DCX Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Doublecortin (DCX) (AA 1-365) protein (Strep Tag)Crystallography grade DCX Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Doublecortin (DCX) (AA 1-366) protein (Strep Tag)Crystallography grade DCX Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Doublecortin (DCX) (AA 1-361), (full length) protein (His tag)DCX Origin: Chicken Host: Escherichia coli (E. coli) Recombinant 90 % SDS, ELISA
Doublecortin (DCX) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)DCX Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Doublecortin (DCX) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)DCX Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "LC-MS/MS identification of doublecortin as abundant beta cell-selective protein discharged by damaged beta cells in vitro." in: Journal of proteomics, Vol. 80, pp. 268-80, (2013) (PubMed).
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: "LC-MS/MS identification of doublecortin as abundant beta cell-selective protein discharged by damaged beta cells in vitro." in: Journal of proteomics, Vol. 80, pp. 268-80, (2013) (PubMed).
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- Target
- Doublecortin (DCX)
- Alternative Name
- DCX (DCX Products)
- Synonyms
- DCX Protein, DBCN Protein, DC Protein, LISX Protein, SCLH Protein, XLIS Protein, Dbct Protein, 18C15.5 Protein, doublecortin Protein, DCX Protein, Dcx Protein
- Background
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Full Gene Name: doublecortin
Synonyms: DBCN,DC,LISX,SCLH,XLIS - Gene ID
- 1641
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