EZH2 Protein (Lys27) (DYKDDDDK Tag,His tag)

Catalog No. ABIN2669606

Product Details

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Lys27

Origin: Human

Source: Baculovirus

Purification tag / Conjugate: This EZH2 protein is labelled with DYKDDDDK Tag,His tag.

Application: Enzyme Activity Assay (EAA)

Characteristics: The EZH2 protein complex, active contains EZH2, EED and Suz12 which are the minimal components required for histone methyltransferase activity. EZH2 is specific for H3 lysine 27, a mark of repressed regions of chromatin. Recombinant human EZH2 (accession number NM_004456, amino acids 2-end with an N-terminal His tag, molecular weight 86 kDa) was coexpressed in a baculovirus expression system along with human EED (accession number NM_003797, amino acids 2-end with an N-terminal FLAG tag, molecular weight 51 kDa) and human Suz12 (accession number NM_015355, amino acids 2-end with an N-terminal His tag, molecular weight 87 kDa).

Application Details

Application Notes: Recombinant EZH2 protein complex is suitable for use in histone methyltransferase assays. This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Specific Activity: 0.08 pmol/min/μg. Assay conditions: Prepare a 50 μL reaction mix (20 mM phosphate pH 7.4, 0.05 % Tween-20, 40 μM S-adenosylmethionine, and 250-2000 ng enzyme complex) and add to substrate-coated wells with the substrate on a Thermo Neutravidin black plate. Incubate for 1 hr. Add anti-methyl histone H3K27 antibody and incubate 1hr. Next, incubate 1h with the HRP-labeled secondary antibody followed by chemiluminescent detection.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2 (EZH2 Products)

Synonyms: ENX-1 Protein, ENX1 Protein, EZH1 Protein, EZH2b Protein, KMT6 Protein, KMT6A Protein, WVS Protein, WVS2 Protein, eya2 Protein, im:7149338 Protein, zgc:136376 Protein, zgc:152758 Protein, EZH2 Protein, enx-1 Protein, enx1 Protein, ezh1 Protein, kmt6 Protein, xez Protein, Enx-1 Protein, Enx1h Protein, mKIAA4065 Protein, ezh2 Protein, olezh2 Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit L homeolog Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit S homeolog Protein, EZH2 Protein, ezh2 Protein, Ezh2 Protein, ezh2.L Protein, ezh2.S Protein

Background: EZH2, EED and Suz12 are present in the PRC protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 - (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Enhancer of Zeste protein. It contains a SET domain that catalyzes the mono-, di- and trimethylation of Histone H3 Lys27. Polycomb group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. Deregulation of EZH2 is linked to a variety of cancers.

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation