GCLC Protein (AA 1-637) (GST tag)
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- Target See all GCLC Proteins
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-637
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This GCLC protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- GCLC (Human) Recombinant Protein (P01)
- Sequence
- MGLLSQGSPL SWEETKRHAD HVRRHGILQF LHIYHAVKDR HKDVLKWGDE VEYMLVSFDH ENKKVRLVLS GEKVLETLQE KGERTNPNHP TLWRPEYGSY MIEGTPGQPY GGTMSEFNTV EANMRKRRKE ATSILEENQA LCTITSFPRL GCPGFTLPEV KPNPVEGGAS KSLFFPDEAI NKHPRFSTLT RNIRHRRGEK VVINVPIFKD KNTPSPFIET FTEDDEASRA SKPDHIYMDA MGFGMGNCCL QVTFQACSIS EARYLYDQLA TICPIVMALS AASPFYRGYV SDIDCRWGVI SASVDDRTRE ERGLEPLKNN NYRISKSRYD SIDSYLSKCG EKYNDIDLTI DKEIYEQLLQ EGIDHLLAQH VAHLFIRDPL TLFEEKIHLD DANESDHFEN IQSTNWQTMR FKPPPPNSDI GWRVEFRPME VQLTDFENSA YVVFVVLLTR VILSYKLDFL IPLSKVDENM KVAQKRDAVL QGMFYFRKDI CKGGNAVVDG CGKAQNSTEL AAEEYTLMSI DTIINGKEGV FPGLIPILNS YLENMEVDVD TRCSILNYLK LIKKRASGEL MTVARWMREF IANHPDYKQD SVITDEMNYS LILKCNQIAN ELCECPELLG SAFRKVKYSG SKTDSSN
- Characteristics
- Human GCLC full-length ORF ( NP_001489.1, 1 a.a. - 637 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
- Top Product
- Discover our top product GCLC Protein
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Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-218) protein (His-IF2DI Tag)
GCLC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 528-637) protein (GST tag)GCLC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-637) protein (Strep Tag)Crystallography grade GCLC Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-637) protein (Strep Tag)Crystallography grade GCLC Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) proteinGCLC Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) proteinGCLC Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) protein (Myc-DYKDDDDK Tag)GCLC Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
- Alternative Name
- GCLC (GCLC Products)
- Synonyms
- GCLC Protein, gclc Protein, MGC146287 Protein, 2259 Protein, CG2259 Protein, DmGCLC Protein, DmGCS Protein, DmGCSh Protein, Dmel\\CG2259 Protein, GCL Protein, GCLc Protein, GCSh Protein, Gcs Protein, Gcsh Protein, DDBDRAFT_0186120 Protein, DDBDRAFT_0231403 Protein, DDB_0186120 Protein, DDB_0231403 Protein, GCS Protein, GLCL Protein, GLCLC Protein, D9Wsu168e Protein, GLCL-H Protein, Ggcs-hs Protein, Glclc Protein, cb1049 Protein, fe36e11 Protein, wu:fe36e11 Protein, glutamate-cysteine ligase catalytic subunit Protein, glutamate-cysteine ligase, catalytic subunit Protein, Glutamate-cysteine ligase catalytic subunit Protein, gamma glutamylcysteine synthetase Protein, glutamate-cysteine ligase, catalytic subunit L homeolog Protein, glutamate-cysteine ligase, catalytic subunit S homeolog Protein, gamma-glutamylcysteine synthetase Protein, GCLC Protein, gclc Protein, Gclc Protein, LbGCS Protein, gcsA Protein, gclc.L Protein, gclc.S Protein, GSH1 Protein
- Background
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Full Gene Name: glutamate-cysteine ligase, catalytic subunit
Synonyms: GCS,GLCL,GLCLC - Gene ID
- 2729
- NCBI Accession
- NM_001498
- Pathways
- Cell RedoxHomeostasis
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