GCLM Protein (AA 1-274) (GST tag)
Quick Overview for GCLM Protein (AA 1-274) (GST tag) (ABIN1354926)
Target
See all GCLM ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-274
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Purification tag / Conjugate
- This GCLM protein is labelled with GST tag.
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Purpose
- GCLM (Human) Recombinant Protein (P01)
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Sequence
- MGTDSRAAKA LLARARTLHL QTGNLLNWGR LRKKCPSTHS EELHDCIQKT LNEWSSQINP DLVREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVES NSSSSTRSAV DMACSVLGVA QLDSVIIASP PIEDGVNLSL EHLQPYWEEL ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL TAFAKQFDIQ LLTHNDPKEL LSGASFQEAL QESIPDIQAH EWVPLWLLRY SVIVKSRGII KSKGYILQAK RRGS
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Characteristics
- Human GCLM full-length ORF ( AAH41809, 1 a.a. - 274 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (His tag)
custom-made GCLM Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (His tag)custom-made GCLM Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (AA 1-274) protein (His tag)GCLM Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) protein (Myc-DYKDDDDK Tag)GCLM Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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- GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))
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Alternative Name
- GCLM
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Background
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Full Gene Name: glutamate-cysteine ligase, modifier subunit
Synonyms: GLCLR -
Gene ID
- 2730
Target
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