Multiple Tag protein (TRX tag,T7 tag,HSV tag,MYC tag,VSV-G tag,Glu-Glu tag,V5 tag,E tag,DYKDDDDK Tag,S tag,HA tag,KT3 tag,E2 tag,AU1 tag,AU5 tag,His tag)
Origin: Tag
DB, WB
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- Target
- Multiple Tag
- Origin
- Tag
- Host
- Please inquire
- Purification tag / Conjugate
- TRX tag,T7 tag,HSV tag,MYC tag,VSV-G tag,Glu-Glu tag,V5 tag,E tag,DYKDDDDK Tag,S tag,HA tag,KT3 tag,E2 tag,AU1 tag,AU5 tag,His tag
- Application
- Dot Blot (DB), Western Blotting (WB)
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- Restrictions
- For Research Use only
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- Reconstitution
- Add 200 µl of ddH2O to make the final concentration of 1 µg/µl in PBS Buffer. 0.04-0.2 µl of Multiple Tag is recommended for each lane of the minigel (for accurate loading, dilute with PBS Buffer).
- Concentration
- 200 µg of purified Multiple Tag (lyophilized)
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the protein at -20°C or below. After reconstitution, aliquot and store at -20°C or below.
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- Target
- Multiple Tag
- Target Type
- Tag
- Background
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Epitope Tag Positive Control Lysates. Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. This fusion protein is of 52 kDa in mass. It contains 16 commonly used tags, which are listed on the table below, from the N-terminus to the C-terminus.
TagSequences:
Trx: MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLA
T7: CMASMTGGQQMG
HSV: QPELAPEDPEDC
c-Myc: EQKLISEEDLC
VSV-g: YTDIEMNRLGK
Glu-Glu: CEEEEYMPME
V5: GKPIPNPLLGLDST
E-tag: GAPVPYPDPLEPR
DY kDaDDDK-tag: DY kDaDDDK
S-tag: KETAAAKFERQHMDS
HA: YPYDVPDYAC
KT3: KPPTPPPEPET
E2: GVSSTSSDFRDR
AU1: DTYRYI
AU5: TDFYLK
6x His: HHHHHH
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