RAD18 Protein (AA 1-487) (His tag)

Catalog No. ABIN1660210

Product Details

Target: RAD18 (Rad18p (RAD18))

Protein Type: Recombinant

Protein Characteristics: AA 1-487

Origin: Saccharomyces cerevisiae

Source: Yeast

Purification tag / Conjugate: This RAD18 protein is labelled with His tag.

Application: ELISA

Sequence: MDHQITTASD FTTTSIPSLY QLDTLLRCHI CKDFLKVPVL TPCGHTFCSL CIRTHLNNQP NCPLCLFEFR ESLLRSEFLV SEIIQSYTSL RSSLLDALRI PKPTPVPENE EVPGPENSSW IELISESESD SVNAADDDLQ IVATSERKLA KRSMTDILPL SSKPSKRNFA MFRSERIKKK SKPNEQMAQC PICQQFYPLK ALEKTHLDEC LTLQSLGKKP KISTTFPTES NPHNKSSSRF KVRTPEVDKS SCGETSHVDK YLNSMMSAEH QRLPKINFTS MTQSQIKQKL SSLGLSTNGT RQNMIKRYNH YEMLWNSNFC DSLEPVDEAE LKRQLLSWDV SHNKTPQNSS NKGGISKLMI MKSNGKSSSY RKLLENFKND KFNRKGWMVM FRKDFARLIR EAKMKIKTGS SDSSGSVGHS NDGDGVEKVQ SDQGTEDQQM EKDQDTVINE DRVAGERNLP NEDSTDADLS RELMDLNEYS KDPPGNN

Specificity: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: RAD18 (Rad18p (RAD18))

Alternative Name: Postreplication repair E3 ubiquitin-protein ligase RAD18 (RAD18) (RAD18 Products)

Synonyms: similar to S. cerevisiae RAD18 (YCR066W) ubiquitin conjugating enzyme involved in DNA repair, Zn finger protein; same as cosmid orf Ca20C1.14c Protein, RAD18 Protein

Background: Recommended name: Postreplication repair E3 ubiquitin-protein ligase RAD18.
EC= 6.3.2.-.
Alternative name(s): Radiation sensitivity protein 18

UniProt: P10862