nectin-3 Protein (C-Term, Extracellular Domain)

Catalog No. ABIN1684642

This Recombinant nectin-3 protein is produced in HEK-293T Cells.

Quick Overview for nectin-3 Protein (C-Term, Extracellular Domain) (ABIN1684642)

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293T Cells

Purity: > 95 %, as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: C-Term, Extracellular Domain

Specificity: Optimized DNA sequence encoding extracellular domain of human CD113 (nectin-3) including a C-terminal 6His tag was expressed in HEK293 cells.

Characteristics: Recombinant CD113 (nectin-3) is a monomer protein consisting of 354 amino acid residue subunits, due to glycosylation migrates as an approximately 70 kDa protein on SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

The activity of Recombinant human CD113 was measured in a functional ELISA assay, immobilized recombinant human CD113 at 1 µg/mL ( 100 µL/well ) can bind biotinylated Nectin-1 with a linear range of 6 - 800 µg/mL

Restrictions: For Research Use only

Handling

Buffer: PBS solution, pH7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C/-80 °C

Storage Comment: Recombinant rat PDGFRb as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.

Expiry Date: 12 months

Target Details

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Alternative Name: CD113

Background: The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (PDGFA, PDGFB, PDGFC, and PDGFD).

UniProt: Q9NQS3

Pathways: Cell-Cell Junction Organization