nectin-3 Protein (AA 58-400) (His-Avi Tag)

Catalog No. ABIN7275311

Recombinant nectin-3 protein expressed in HEK-293 Cells.

Quick Overview for nectin-3 Protein (AA 58-400) (His-Avi Tag) (ABIN7275311)

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 58-400

Purification tag / Conjugate: This nectin-3 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Nectin-3/CD113 Protein

Sequence: Gly58-Asp400

Characteristics: Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly58-Asp400.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).,Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test).,Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM PB, 250 mM NaCl ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Alternative Name: Nectin-3

Background: The Nectin family has at least four members (Nectin-1-4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.

Molecular Weight: 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Bis-Tris PAGE result.

UniProt: Q9NQS3

Pathways: Cell-Cell Junction Organization