BDH1 Protein (AA 1-343) (His tag)

Catalog No. ABIN1691949

Product Details

Target: BDH1 (3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1))

Protein Type: Recombinant

Protein Characteristics: AA 1-343

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BDH1 protein is labelled with His tag.

Purpose: Recombinant Human D-β-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MLATRLSRPL SRLPGKTLSA CDRENGARRP LLLGSTSFIP IGRRTYASAA EPVGSKAVLV TGCDSGFGFA LAKHLHSKGF LVFAGCLMKD KGHDGVKELD SLNSDRLRTV QLNVCSSEEV EKVVEIVRSS LKDPEKGMWG LVNNAGISTF GEVEFTSLET YKQVAEVNLW GTVRMTKSFL PLIRRAKGRV VNISSMLGRM ANPARSPYCI TKFGVEAFSD CLRYEMYPLG VKVSVVEPGN FIAATSLYSP ESIQAIAKKM WEELPEVVRK DYGKKYFDEK IAKMETYCSS GSTDTSPVID AVTHALTATT PYTRYHPMDY YWWLRMQIMT HLPGAISDMI YIR

Characteristics: Recombinant Human D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Arg343) of Human BDH1 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 1 mM DTT, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: BDH1 (3-hydroxybutyrate Dehydrogenase, Type 1 (BDH1))

Alternative Name: D-beta-Hydroxybutyrate Dehydrogenase Mitochondrial/BDH1 (BDH1 Products)

Synonyms: zgc:163053 Protein, BDH Protein, SDR9C1 Protein, 2310032J20Rik Protein, AI327223 Protein, Bdh Protein, 3-hydroxybutyrate dehydrogenase 1 Protein, 3-hydroxybutyrate dehydrogenase 1 S homeolog Protein, 3-hydroxybutyrate dehydrogenase, type 1 Protein, 3-hydroxybutyrate dehydrogenase, type 1 A Protein, BDH1 Protein, bdh1.S Protein, bdh1 Protein, Bdh1 Protein, BDH1A Protein

Sub Type: Fusionprotein

Background: D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial (BDH) is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH is localized in the mitochondrion matrix. BDH forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism.
Alternative Names: D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial, BDH, 3-Hydroxybutyrate Dehydrogenase, BDH

Molecular Weight: 40.3 kDa

UniProt: Q02338

Pathways: Response to Growth Hormone Stimulus