BPGM Protein (AA 2-259) (His tag)

Catalog No. ABIN1692046

Product Details

Target: BPGM (2,3-bisphosphoglycerate Mutase (BPGM))

Protein Type: Recombinant

Protein Characteristics: AA 2-259

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BPGM protein is labelled with His tag.

Purpose: Recombinant Human Bisphosphoglycerate Mutase/BPGM (C-6His)

Sequence: SKYKLIMLRH GEGAWNKENR FCSWVDQKLN SEGMEEARNC GKQLKALNFE FDLVFTSVLN RSIHTAWLIL EELGQEWVPV ESSWRLNERH YGALIGLNRE QMALNHGEEQ VRLWRRSYNV TPPPIEESHP YYQEIYNDRR YKVCDVPLDQ LPRSESLKDV LERLLPYWNE RIAPEVLRGK TILISAHGNS SRALLKHLEG ISDEDIINIT LPTGVPILLE LDENLRAVGP HQFLGDQEAI QAAIKKVEDQ GKVKQAKKLE HHHHHH

Characteristics: Recombinant Human Bisphosphoglycerate Mutase/BPGM is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys259) of Human BPGM fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris, 1 mM DTT, pH 8.0 .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: BPGM (2,3-bisphosphoglycerate Mutase (BPGM))

Alternative Name: Bisphosphoglycerate Mutase/BPGM (BPGM Products)

Synonyms: DPGM Protein, AI323730 Protein, AL022789 Protein, C86192 Protein, Ab2-098 Protein, zgc:92230 Protein, Bisphosphoglycerate mutase Protein, bisphosphoglycerate mutase Protein, 2,3-bisphosphoglycerate mutase Protein, bisphosphoglycerate mutase S homeolog Protein, pmge Protein, BPGM Protein, Bpgm Protein, bpgm Protein, bpgm.S Protein

Sub Type: Fusionprotein

Background: Bisphosphoglycerate Mutase (BPGM) is a member of the Phosphoglycerate Mutase family and BPG-Dependent PGAM subfamily. BPGM is a multifunctional enzyme. BPGM catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. It also has phosphoglycerate phosphomutase activity. BPGM plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Deficiency of BPGM increases the affinity of cells for oxygen and result in hemolytic anemia.
Alternative Names: Bisphosphoglycerate Mutase, BPGM, 2,3-Bisphosphoglycerate Mutase Erythrocyte, 2,3-Bisphosphoglycerate Synthase, 2,3-Diphosphoglycerate Mutase, DPGM, BPG-Dependent PGAM, BPGM

Molecular Weight: 31 kDa

UniProt: P07738