Caspase 10 Protein (AA 220-480) (His tag)

Catalog No. ABIN1692217

Product Details

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Protein Type: Recombinant

Protein Characteristics: AA 220-480

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Caspase 10 protein is labelled with His tag.

Purpose: Recombinant Human Caspase-10/CASP10 (C-6His)

Sequence: MVKTFLEALP QESWQNKHAG SNGNRATNGA PSLVSRGMQG ASANTLNSET STKRAAVYRM NRNHRGLCVI VNNHSFTSLK DRQGTHKDAE ILSHVFQWLG FTVHIHNNVT KVEMEMVLQK QKCNPAHADG DCFVFCILTH GRFGAVYSSD EALIPIREIM SHFTALQCPR LAEKPKLFFI QACQGEEIQP SVSIEADALN PEQAPTSLQD SIPAEADFLL GLATVPGYVS FRHVEEGSWY IQSLCNHLKK LVPRHEDILS ILEHHHHHH

Characteristics: Recombinant Human Caspase-10/CASP10 is produced with our E. coli expression system. The target protein is expressed with sequence (Val220 ­ Ile479) of Human CASP10 fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 25 mM HEPES, 10 mM DTT, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Alternative Name: Caspase-10/CASP10 (CASP10 Products)

Synonyms: xCaspase-10 Protein, alps2 Protein, caspace-10 Protein, caspase-10 Protein, flice2 Protein, mch4 Protein, xCaspace-10 Protein, ALPS2 Protein, FLICE2 Protein, MCH4 Protein, caspase 10 S homeolog Protein, caspase 10 Protein, caspase-10 Protein, casp10.S Protein, casp10 Protein, CASP10 Protein, Casp10 Protein, LOC100848685 Protein

Sub Type: Fusionprotein

Background: Caspase-10 (CASP10) is a 521 amino acid protein member of the Cysteine-Aspartic Acid Protease (Caspase) family. CASP10 contains two DED (Death Effector) domains and is detectable in most tissues. CASP10 cleavage by Granzyme B and autocatalytic activity generate the two active subunits: Caspase-10 subunit p23/17, Caspase-10 subunit p12. Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the execution-phase of cell apoptosis, the initiation and execution. Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7). CASP10 cleaves and activates caspases 3 and 7, but itself is processed by caspase 8. Defects in CASP10 are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome.
Alternative Names: Caspase-10, CASP-10, Apoptotic Protease Mch-4, FAS-Associated Death Domain Protein Interleukin-1B-Converting Enzyme 2, FLICE2, ICE-Like Apoptotic Protease 4, CASP10, MCH4

Molecular Weight: 30.1 kDa

Pathways: Apoptosis, Caspase Cascade in Apoptosis