Caspase 10 Protein (His tag)

Catalog No. ABIN7318249

Product Details

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Caspase 10 protein is labelled with His tag.

Purpose: Recombinant Human Caspase-10/CASP10 Protein (His Tag)

Sequence: Val220-Ile480

Characteristics: Recombinant Human Caspase-10 is produced by our E.coli expression system and the target gene encoding Val220-Ile480 is expressed with a 6His tag at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 25 mM HEPES, 10 mM DTT, pH 7.5.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Alternative Name: Caspase-10/CASP10 (CASP10 Products)

Synonyms: xCaspase-10 Protein, alps2 Protein, caspace-10 Protein, caspase-10 Protein, flice2 Protein, mch4 Protein, xCaspace-10 Protein, ALPS2 Protein, FLICE2 Protein, MCH4 Protein, caspase 10 S homeolog Protein, caspase 10 Protein, caspase-10 Protein, casp10.S Protein, casp10 Protein, CASP10 Protein, Casp10 Protein, LOC100848685 Protein

Background:

Background: Caspase-10 (CASP10) is a 521 amino acid protein member of the Cysteine-Aspartic Acid Protease (Caspase) family. CASP10 contains two DED (Death Effector) domains and is detectable in most tissues. CASP10 cleavage by Granzyme B and autocatalytic activity generate the two active subunits: Caspase-10 subunit p23/17, Caspase-10 subunit p12. Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the execution-phase of cell apoptosis, the initiation and execution. Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7). CASP10 cleaves and activates caspases 3 and 7, but itself is processed by caspase 8. Defects in CASP10 are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome.

Synonym: Caspase-10, CASP-10, Apoptotic Protease Mch-4, FAS-Associated Death Domain Protein Interleukin-1B-Converting Enzyme 2, FLICE2, ICE-Like Apoptotic Protease 4, CASP10, MCH4

Molecular Weight: 30.1 kDa

Pathways: Apoptosis, Caspase Cascade in Apoptosis