LacI (AA 1-360) protein (His tag)
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- LacI
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-360
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Sequence
- MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH
- Characteristics
- Recombinant E.coli Lactose operon repressor/LacI is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gln360) of E.coli LacI fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 5 mM DTT pH 8.0 .
- Handling Advice
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Always centrifuge tubes before opening.
Do not mix by vortex or pipetting.
Please aliquot to minimize freeze-thaw cycles. - Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- LacI
- Synonyms
- ECK0342 Protein, JW0336 Protein, lactose-inducible lac operon transcriptional repressor Protein, lacI Protein
- Sub Type
- Fusionprotein
- Background
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Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
Alternative Names: Lactose operon repressor, LacI - Molecular Weight
- 39.4 kDa
- UniProt
- P03023
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