TRIM5 Protein (AA 1-248) (His tag)

Catalog No. ABIN1945366

Product Details

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Protein Type: Recombinant

Protein Characteristics: AA 1-248

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TRIM5 protein is labelled with His tag.

Purpose: Recombinant Human Tripartite Motif-Containing Protein 5/TRIM5/RNF88 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MASGILVNVK EEVTCPICLE LLTQPLSLDC GHSFCQACLT ANHKKSMLDK GESSCPVCRI SYQPENIRPN RHVANIVEKL REVKLSPEGQ KVDHCARHGE KLLLFCQEDG KVICWLCERS QEHRGHHTFL TEEVAREYQV KLQAALEMLR QKQQEAEELE ADIREEKASW KTQIQYDKTN VLADFEQLRD ILDWEESNEL QNLEKEEEDI LKSLTNSETE MVQQTQSLRE LISDLEHRLQ GSVMELLQ

Characteristics: Recombinant Human TRIM5 is produced by our E. coli expression system. The target protein is expressed with sequence (AA 1-248) of Human TRIM5 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: Tripartite Motif-Containing Protein 5 (TRIM5 Products)

Synonyms: TRIM5 Protein, RNF88 Protein, TRIM5alpha Protein, EG667823 Protein, Gm8833 Protein, RGD1304579 Protein, tripartite motif containing 5 Protein, tripartite motif-containing 5 Protein, TRIM5 Protein, Trim5 Protein

Background: Tripartite motif-containing Motif 5 is a protein that in humans is encoded by the TRIM5 gene.It is a 493 amino acids protein that belongs to the TRIM/RBCC family.It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM5 present in the cytoplasm recognizes motifs within the capsid proteins and interferes with the uncoating process, therefore preventing successful reverse transcription and transport to the nucleus of the viral genome. The exact mechanism of action has not been shown conclusively, but capsid protein from restricted viruses is removed by proteasome-dependent degradation

Molecular Weight: 30.8 kDa

UniProt: Q9C035

Pathways: Activation of Innate immune Response