FGF8 Protein (AA 23-204, Isoform A)

Catalog No. ABIN2017792

Product Details

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-204, Isoform A

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 500 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 1 μg/mL of heparin, corresponding to a specific activity of > 2x 10^3 units/mg.
AA 23-204 (isoform a), expressed with an N-terminal Met.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Fibroblast Growth Factor 8a (rhFGF-8a) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF-8a remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF8 (Fibroblast Growth Factor 8 (Androgen-Induced) (FGF8))

Abstract: FGF8 Products

Synonyms: Aigf Protein, Fgf-8 Protein, AIGF Protein, FGF-8 Protein, HBGF-8 Protein, HH6 Protein, KAL6 Protein, fibroblast growth factor 8 Protein, Fgf8 Protein, FGF8 Protein

Background: Fibroblast Growth Factor 8a (FGF-8a) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. FGF-8 has 8 different isoforms, named FGF-8a through FGF-8h. Different FGF-8 isoforms have different affinities to the receptors, and thus participate in different signaling cascade pathways. FGF-8 has very widespread expression during embryonic development, and is an organizer and inducer for gastrulation, somitogenesis, morphogenesis, and limb induction. However, FGF-8 is also a potential oncogene: in normal adult cells, FGF-8 has very low expression, but FGF-8 is highly expressed in cancer cells of breast, prostate, and ovarian tumors. FGF-8 promotes tumor angiogenesis by increasing neovascularization, and induces osteoblastic differentiation. Recombinant human Fibroblast Growth Factor 8a (rhFGF-8a) produced inE. coli is a single non-glycosylated polypeptide chain containing 183 amino acids. A fully biologically active molecule, rhFGF-8a has a molecular mass of 21.3 kDa analyzed by reducing SDS-PAGE.
Synonyms: AIGFa, HBGF-8a

Molecular Weight: 21.3 kDa, observed by reducing SDS-PAGE.

UniProt: P55075

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis