IL12 Protein (AA 23-215, AA 23-335)
Quick Overview for IL12 Protein (AA 23-215, AA 23-335) (ABIN2018058)
Target
See all IL12 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 23-215, AA 23-335
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Characteristics
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ED50 < 0.15 ng/mL, measured in a cell proliferation assay using 2D6 cells.
AA 23-215 (p35) and AA 23-335 (p40), expressed with C-terminal 6xHis. -
Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
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Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag)
IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Interleukin 12 (IL12) (AA 23-328) protein (His-Avi Tag,Biotin)IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) proteinIL12 Origin: Human Host: HEK-293T Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 12 (IL12) (AA 23-215) protein (His tag)IL12 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,DYKDDDDK Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)IL12 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-219) protein (Fc-His Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 12 (IL12) proteinIL12 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE
Interleukin 12 (IL12) (Active) protein (His tag)IL12 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS, AcA Active
Interleukin 12 (IL12) proteinIL12 Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstituted in ddH2O or PBS at 100 μg/mL.
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Buffer
- Lyophilized after extensive dialysis against PBS.
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Storage
- -80 °C
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Storage Comment
- Lyophilized recombinant murineInterleukin-12 (IL-12), remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, murine Interleukin-12 (IL-12), His should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.
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Expiry Date
- 6 months
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- IL12 (Interleukin 12 (IL12))
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Alternative Name
- Interleukin-12 (IL-12)
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Background
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Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals throughtheIL-12 receptor complex, which is comprised of IL-12 Rbeta1 and IL-12 Rbeta2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
Synonyms: NKSF, TCMF, CLMF, TSF -
Molecular Weight
- 27-29 kDa (p35) and 45-50 kDa (p40), observed by reducing SDS-PAGE.
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UniProt
- P43431, P43432
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Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target
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