IL-3 beta Protein (AA 27-169)
IL-3 beta
Origin: Rat
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as analyzed by SDS-PAGE and HPLC.
Active
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- Target See all IL-3 beta products
- IL-3 beta (Interleukin 3 beta (IL-3 beta))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 27-169
- Origin
- Rat
- Source
- Escherichia coli (E. coli)
- Characteristics
-
ED50 < 5 ng/mL, measured by a cell proliferation assay using NFS-60 cells, corresponding to a specific activity of > 2 x 10^5 units/mg.
AA 27-169, expressed with an N-terminal Met. - Purity
- > 95 % as analyzed by SDS-PAGE and HPLC.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant rat Interleukin-3 beta (rrIL-3 beta) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrIL-3 beta remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- IL-3 beta (Interleukin 3 beta (IL-3 beta))
- Abstract
- IL-3 beta Products
- Background
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Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific alpha chain and a common beta chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family. Recombinant rat Interleukin-3 beta (rrIL-3 beta) produced in E. coli is a single non-glycosylated polypeptide chain containing of 144 amino acids. A fully biologically active molecule, rrIL-3 beta has a molecular mass of 16.3 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor - Molecular Weight
- 16.3 kDa, observed by non-reducing SDS-PAGE.
- UniProt
- P97688
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