Cathepsin G (CTSG) Protein

Details for Product No. ABIN2128355, Supplier: Log in to see
Protein Name
  • CATG
  • CG
  • CTSG
  • Ctsg
  • cathepsin G
  • CTSG
  • Ctsg
  • LOC505658
  • LOC509956
  • ctsg
  • LOC100053921
  • LOC102174648
  • LOC100733619
  • LOC100154047
Origin
Human
16
4
2
Source
Human
10
7
2
2
1
Protein Type
Native
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'Independent Validation' Badge
Antigen CTSG
Lot Number A14041801
Method validated Unfolding Profile
Positive Control ABIN2128355
Notes

Passed. ABIN2128355 showed a Ti of 63.8 °C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

Protocol
  • Dilute ABIN2128355 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.5µM.
  • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
  • Run Tycho measurement.
Experimental Notes

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Validation Images
Unfolding Profile validation image for Cathepsin G (CTSG) protein (ABIN2128355) Unfolding profile of ABIN2128355. The fluorescence signal is plotted against temperat...
Purity > 96 % pure
Protein Name
Pathways ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Liquid 50 mM sodium acetate, pH 5.5, 0.8 M sodium chloride
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