POMC Protein (His tag)
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- Target See all POMC Proteins
- POMC (Proopiomelanocortin (POMC))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POMC protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- 27-267 amino acids: MGSSHHHHHH SSGLVPRGSH MWCLESSQCQ DLTTESNLLE CIRACKPDLS AETPMFPGNG DEQPLTENPR KYVMGHFRWD RFGRRNSSSS GSSGAGQKRE DVSAGEDCGP LPEGGPEPRS DGAKPGPREG KRSYSMEHFR WGKPVGKKRR PVKVYPNGAE DESAEAFPLE FKRELTGQRL REGDGPDGPA DDGAGAQADL EHSLLVAAEK KDEGPYRMEH FRWGSPPKDK RYGGFMTSEK SQTPLVTLFK NAIIKNAYKK GE
- Characteristics
- Recombinant human POMC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Purity
- > 85 % pure
- Top Product
- Discover our top product POMC Protein
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Proopiomelanocortin (POMC) (AA 205-267) protein (GST tag)
POMC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Proopiomelanocortin (POMC) (AA 27-267) protein (His tag)POMC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Proopiomelanocortin (POMC) (AA 27-267) protein (His-IF2DI Tag)POMC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Proopiomelanocortin (POMC) (AA 1-267) protein (GST tag)POMC Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Proopiomelanocortin (POMC) proteinPOMC Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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- Application Notes
- POMC protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 1 mM DTT, 50 % glycerol and 0.2M NaCl.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
- Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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- Target
- POMC (Proopiomelanocortin (POMC))
- Alternative Name
- POMC (POMC Products)
- Synonyms
- MSH Protein, ACTH Protein, pomc Protein, SI:bZ36D5.1 Protein, SI:bZ36D5.4 Protein, si:bz36d5.2 Protein, BE Protein, Beta-LPH Protein, Clip Protein, Gamma-LPH Protein, Npp Protein, Pomc-1 Protein, Pomc1 Protein, alpha-MSH Protein, alphaMSH Protein, beta-MSH Protein, gamma-MSH Protein, CLIP Protein, LPH Protein, NPP Protein, POC Protein, Pomc2 Protein, proopiomelanocortin a Protein, pro-opiomelanocortin-alpha Protein, proopiomelanocortin Protein, pomca Protein, Pomc Protein, POMC Protein
- Background
- Pro-opiomelanocortin preproprotein, also known as POMC, a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. It regulates the corticosteroid production in the adrenal cortex. Also, POMC is cleaved into ten hormone chains named NPP, gamma-MSH, ACTH, alpha-MSH, CLIP, Lipotropin beta, Lipotropin gamma, Beta-MSH, Beta-endorphin and Met-enkephalin. Defects in the gene that encodes POMC are the cause of POMC deficiency, which is characterized by red hair and adrenal insufficiency.
- Molecular Weight
- 28.9 kDa (262aa), confirmed by MALDI-TOF(molecular weight on SDS-PAGE will appear higher)
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour
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