CD55 Protein (AA 35-353) (His tag)

Catalog No. ABIN2180811

Product Details

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Protein Type: Recombinant

Protein Characteristics: AA 35-353

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD55 protein is labelled with His tag.

Sequence: AA 35-353

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.8 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

References

Kwon, Kim, Meyer, Di Bisceglie, Ray: "Distinct CD55 Isoform Synthesis and Inhibition of Complement-Dependent Cytolysis by Hepatitis C Virus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 197, Issue 4, pp. 1127-36, (2017) (PubMed).

Target Details

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 (CD55 Products)

Synonyms: CR Protein, CROM Protein, DAF Protein, TC Protein, Daf Protein, Daf-GPI Protein, Daf1 Protein, GPI-DAF Protein, CD55 molecule (Cromer blood group) Protein, CD55 molecule, decay accelerating factor for complement Protein, CD55 Protein, Cd55 Protein

Background: CD55 is also known as DAF or decay-accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first SCR. It also binds physiologically-generated C3 convertases with its second and third SCRs. Binding results in an accelerated ""decay"", or dissociation of active C3 convertases, thus blocking the development of C' attack complexes on nonforeign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.

Molecular Weight: 35.8 kDa

NCBI Accession: NP_000565

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity