Glycoprotein / GP (Virus) (AA 33-304) protein (His tag)

Catalog No. ABIN2181010

Product Details

Target: Glycoprotein / GP (Virus)

Protein Type: Recombinant

Protein Characteristics: AA 33-304

Origin: Ebola Virus

Source: HEK-293 Cells

Purification tag / Conjugate: His tag

Sequence: AA 33-304

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 31.8 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE).

Purity: >90 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Smiley Evans, Tutaryebwa, Gilardi, Barry, Marzi, Eberhardt, Ssebide, Cranfield, Mugisha, Mugisha, Kellermann, Mazet, Johnson: "Suspected Exposure to Filoviruses Among People Contacting Wildlife in Southwestern Uganda." in: The Journal of infectious diseases, Vol. 218, Issue suppl_5, pp. S277-S286, (2018) (PubMed).

Target Details

Target: Glycoprotein / GP (Virus)

Target Type: Viral Protein

Background: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160- kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

Molecular Weight: 31.8 kDa