Glycoprotein / GP (Virus) (partial) protein (His tag)
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- Target
- Glycoprotein / GP (Virus)
- Protein Type
- Recombinant
- Protein Characteristics
- partial
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Origin
- Ebola Virus, Bundibugyo Virus (BDBV)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag
- Application
- Western Blotting (WB)
- Sequence
- Ile 33 - Arg 324
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Endotoxin level is less than 1.0 EU per ug by the LAL method.
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Glycoprotein / GP (Virus) (AA 33-304) protein (His tag)
Origin: Ebola Virus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
Glycoprotein / GP (Virus) (AA 67-297) protein (His tag)Origin: Human Respiratory Syncytial Virus (hRSV) Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
Glycoprotein / GP (Virus) (AA 33-501) protein (His tag)Origin: Ebola Virus Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Glycoprotein / GP (Virus) (AA 33-324) protein (His tag)Origin: Ebola Virus Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Glycoprotein / GP (Virus) (AA 33-637) protein (His tag)Origin: Ebola Virus Host: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE.
Glycoprotein / GP (Virus) (AA 23-700) protein (Fc Tag)Origin: Human Herpesvirus 5 (HHV-5) Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
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- Application Notes
- This recombinant protein can be used for WB.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.
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- Target
- Glycoprotein / GP (Virus)
- Target Type
- Viral Protein
- Background
- EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160- kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
- Molecular Weight
- 34.3 kDa
- Gene ID
- 9487265
- UniProt
- B8XCN1
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