Glycoprotein / GP (Virus) (AA 33-637) protein (His tag)

Catalog No. ABIN2870779

Recombinant Glycoprotein / GP (Virus) protein expressed in HEK-293 Cells.

Quick Overview for Glycoprotein / GP (Virus) (AA 33-637) protein (His tag) (ABIN2870779)

Target: Glycoprotein / GP (Virus)

Protein Type: Recombinant

Origin: Ebola Virus

Source: HEK-293 Cells

Purity: >85 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 33-637

Purification tag / Conjugate: His tag

Sequence: AA 33-637

Characteristics: Ebolavirus EBOV(subtype Zaire,strain Kikwit-95) GP, containing GP1 and GP2, is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 67 kDa. The predicted N-terminus is Ile 33. The reducing (R) protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) in SDS-PAGE .

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: Glycoprotein / GP (Virus)

Target Type: Viral Protein

Background: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160- kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

Molecular Weight: 67.2 kDa