Endonuclease G Protein (ENDOG) (AA 51-140) (His tag,Strep II tag)

Catalog No. ABIN2468369

Product Details

Target: Endonuclease G (ENDOG)

Protein Type: Recombinant

Protein Characteristics: AA 51-140

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Endonuclease G protein is labelled with His tag,Strep II tag.

Application: Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purity: ~95 %

Application Details

Application Notes: This recombinant protein can be used for WB, ELISA, MS.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1X PBS containing 0.1 % SDS and 0.02 % NaN

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -80 °C

Storage Comment: Store in working aliquots at -70 °C.

Target Details

Target: Endonuclease G (ENDOG)

Alternative Name: EndoG (ENDOG Products)

Synonyms: CG8862 Protein, Dmel\\CG8862 Protein, Tb08.10J17.140 Protein, Tb08.10J17.90 Protein, TBC1D13 Protein, endog Protein, ENDOG Protein, wu:fb79c08 Protein, zgc:110020 Protein, Endonuclease G Protein, endonuclease G Protein, putative endonuclease G Protein, endonuclease G, mitochondrial Protein, endonuclease G L homeolog Protein, EndoG Protein, Tc00.1047053506867.10 Protein, Tb927.8.4040 Protein, Tb927.8.4090 Protein, LBRM_10_0750 Protein, LMJF_10_0610 Protein, NAEGRDRAFT_78507 Protein, ENDOG Protein, endog Protein, eNDOG Protein, LOC100529218 Protein, Endog Protein, endog.L Protein

Background: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1-3). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndoG also cleaves DNA in vitro (4).

Molecular Weight: 15 kDa (Calculated)

Gene ID: 2021

OMIM: 24638471

UniProt: Q14249

Pathways: Apoptosis